CCL3/Macrophage Inflammatory Protein-1α Is Dually Involved in Parasite Persistence and Induction of a TNF- and IFNγ-Enriched Inflammatory Milieu in Trypanosoma cruzi-Induced Chronic Cardiomyopathy.
CCL3/Macrophage Inflammatory Protein-1α Is Dually Involved in Parasite Persistence and Induction of a TNF- and IFNγ-Enriched Inflammatory Milieu in Trypanosoma cruzi-Induced Chronic Cardiomyopathy.
CCL3, member of the CC-chemokine family, has been linked to heart tissue macrophage recruitment and parasite control in mouse acute infection with Trypanosoma cruzi, the causative agent of Chagas disease. Here, we approached CCL3 participation in chronic chagasic cardiomyopathy (CCC), the main clinical forms of Chagas disease. We CCC induced in C57BL / 6 (CCL + / +) and CCL3-deficiency (CCL – / -) mice by infection with Colombia Type I strain. In CCL + / + mice, high levels CCL3 mRNA and protein were detected in heart tissue during acute and chronic infections. Survival is not affected by the shortage CCL3. Compared with CCL + / +, chronically infected CCL – / – mice presented decreased cardiac parasitism and inflammation caused by CD8 + cells and macrophages. Leukocytosis decreased in infected CCL – / – mice, in line with the accumulation of CD8 + T cells with no CCR5 + activated LFA-1 + cells in the spleen.
Furthermore, T. cruzi-infected CCL – / – mice served to reduce the frequency of interferon-gamma (IFN) + cells and the number of cell-specific IFN-producing parasite, while the cytotoxic activity of T. cruzi-specific antigen increases. CCL3-deficient macrophages stimulation with IFN improve control of parasites in the environment by reducing nitrous oxides (NOx) and tumor necrosis factor (TNF), but similar to interleukin-10 (IL-10), concentration. Compared to chronic T. cruzi-infected CCL + / + counterparts, CCL – / – mice did not show an enlarged heart, the loss of left ventricular ejection fraction, QTc prolongation and increased activity of CK-MB. Compared with CCL + / +, CCL infected – / – mice showed a decrease in the concentration of TNF, while IL-10 levels were not affected, in the heart of the neighborhood. In the spleen of CCL + / + control NI, most of CD8 + T-cells expressing CCR1 or CCR5 receptor CCL3 that IL-10 +, whereas in infected mice these cells mainly TNF +.
Lastly, blockage selective CCR1 / CCR5 (therapy Met-RANTES) in chronically infected CCL + / + mice reversed electrical abnormalities important (bradycardia, prolonged PR and QTc interval), in correlation with reduced TNF and, especially, the level of CCL3 in liver tissue , Therefore, in T. cruzi infection chronic CCL3 take part in the parasite persistence and contribute to shaping the CD8 + T-cell and macrophage inflammatory enriched heart. Furthermore, increased levels of CCL3 create scenarios with abundant IFN and TNF, is associated with cardiomyocyte injury, cardiac dysfunction and QTc prolongation, a biomarker of the severity of heart disease Chagas’.
CCL3/Macrophage Inflammatory Protein-1α Is Dually Involved in Parasite Persistence and Induction of a TNF- and IFNγ-Enriched Inflammatory Milieu in Trypanosoma cruzi-Induced Chronic Cardiomyopathy.
High-fat GAIT element-mediated translational silencing of diet-induced mRNA that encodes the macrophage inflammatory proteins protect against atherosclerosis.
Previously, we identified the control mechanisms of inflammation directed by ribosomal protein L13a and “GAIT” (Gamma Active Inhibitor of Translation) elements in the mRNA targets and demonstrated that elimination in the myeloid cell-specific L13a KO mice (L13a KO) increases susceptibility to atherosclerosis and severity , Here, we investigate the mechanistic basis of this endogenous defense against atherosclerosis. We compared the molecular and cellular aspects of atherosclerosis in high fat diet (HFD) -fed L13a KO and intact (control) mice. HFD treatment induced rat control L13a release of the 60S ribosome, the formation of RNA-binding complex and further GAIT element-mediated translational silencing. atherosclerotic plaque from HFD-treated KO mice showed infiltration of inflammatory types of M1 macrophages increased.
Description: Recombinant MIP-1 beta is a disulfide-linked homodimeric protein consisting of 70 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human MIP-1 beta chain was expressed in E. coli.
Description: Recombinant MIP-1 beta is a disulfide-linked homodimeric protein consisting of 70 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human MIP-1 beta chain was expressed in E. coli.
Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody
Description: Macrophage Inflammatory Protein-1 alpha Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7820 Dalton. ;The MIP-1a is purified by proprietary chromatographic techniques.
Bovine Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Bovine Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Chicken Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Chicken Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Chicken Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Chicken Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Porcine Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Porcine Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rabbit Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rabbit Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Monkey Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Monkey Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Monkey Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Monkey Macrophage Inflammatory Protein 1 Beta (MIP1b) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Bovine Macrophage Inflammatory Protein 1 Beta (MIP1b)ELISA Kit
Macrophages from knockout mice showed increased phagocytic activity and elevated LDL receptor expression and pro-inflammatory mediators. NanoString analysis of plaque from KO mice showed upregulation of mRNA that encodes a number of inflammatory proteins. bioinformatics analysis showed potential GAIT elements in 3’UTRs some of this mRNA.
