Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation.
Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation.
Chronic inflammation of the adipose tissue contributes to obesity induced insulin resistance. Unfortunately, the potential molecular mechanisms of systemic inflammation and obesity-related metabolic disorders remain complicated. Here, we report that is not active rhomboid-like protein 2 (iRhom2) is increased in obese mice with adipose inflammation.Mice with the elimination of background iRhom2 in C57BL / 6J mice without this gene deletion (control), and mice deficient in only iRhom2 on myeloid cells were fed standard chow diet (SCD) or a high-fat diet (HFD; 60% fat calories).
Then the adipose tissue or bone marrow cells were isolated for more detection.After 16 weeks on a high fat diet (HFD), obesity, chronic inflammation in adipose tissue and insulin resistance were markedly reduced in iRhom2 KO (iRhom2 KO) mice, whereas these parameters exaggerated -besarkan in iRhom2 overactive mice. Adverse effects of iRhom2 in adipose inflammation and related pathologies are determined in db / db mice. We further show that, in response to an HFD mice, iRhom2 knockouts and mice with deletion only in myeloid cells showed less severe inflammation of the adipose tissue and insulin resistance compared to the control group.
By contrast, transplantation of bone marrow cells from normal mice KO mice iRhom2 severe systemic release of inflammatory and metabolic dysfunction after HFD ingestion.We iRhom2 identified as a key regulator that promotes obesity-related metabolic disorders. IRhom2 loss of macrophages in adipose tissue indirectly restrain inflammation and insulin resistance by blocking crosslinks between macrophages and adipocytes. Therefore, iRhom2 may be a therapeutic target for obesity-induced metabolic dysfunction.
Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation.
Changes in macrophage inflammatory protein-1 (MIP-1) expression family members caused by traumatic brain injury in rats.
A deep knowledge of the profound immunological response caused by traumatic brain injury (TBI) raises the possibility of new therapeutic interventions. No studies have highlighted the important role of C-C ligand motif in the development of nerve inflammation after brain injury;
However, the participation of family members macrophage inflammatory protein-1 (MIP-1) in this phenomenon is still undefined. Therefore, the aim of our study was to evaluate changes in inflammatory protein-1 macrophages (MIP-1) family (CCL3, CCl4, and CCL9) and their receptors (CCR1 and CCR5) in a mouse model of TBI (induced by controlled impact cortical (CCI)). We also investigated the patterns of activation of immunological cells (such as neutrophils, microglia and astroglia), which on the one hand express CCR1 / CCR5, and on the other hand may be a source of chemokines tested in brain injury.
We investigated the changes in mRNA (RT-qPCR) and / or protein (ELISA and Western blot) expression in brain structures (cortex, hippocampus, thalamus, and striatum) at points different time (24 hours, 4 days, 7 days, 2 weeks, and / or 5 weeks) after trauma. Studies of time-course revealed upregulation of mRNA expression of all members of MIP-1 family (CCL3, CCl4, and CCL9) in all tested brain structures, especially in the early stages after injury.
Macrophage Inflammatory protein-1 gamma (CCL9) Protein
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Macrophage Inflammatory Protein 1 Gamma ELISA Kit (MIP1g)
Description: MIP-1 gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 101 amino acids and having a molecular mass of 11.6 kDa. The MIP-1 gamma is purified by proprietary chromatographic techniques.
MIP-1a Macrophage Inflammatory Protein-1 Alpha Mouse Recombinant Protein (CCL3)
Description: Macrophage Inflammatory Protein-1 alpha Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7820 Dalton. ;The MIP-1a is purified by proprietary chromatographic techniques.
ELISA kit for Rat MIP1g (Macrophage Inflammatory Protein 1 Gamma)
Description: A sandwich ELISA kit for detection of Macrophage Inflammatory Protein 1 Gamma from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Mouse MIP1g (Macrophage Inflammatory Protein 1 Gamma)
Description: A sandwich ELISA kit for detection of Macrophage Inflammatory Protein 1 Gamma from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Macrophage Inflammatory Protein 1 Gamma (MIP1g) Polyclonal Antibody (Mouse), APC
Description: Macrophage Inflammatory Protein-5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 92 amino acids and having a molecular mass of 10.1 kDa. ;The MIP5 is purified by proprietary chromatographic techniques.
Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g).
Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g).
Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g).
Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g).
Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in samples from n/a with no significant corss-reactivity with analogues from other species.
Macrophage Inflammatory Protein 1 Gamma (MIP1g) Polyclonal Antibody (Mouse), APC-Cy7
Description: Recombinant MIP-1 alpha is a disulfide-linked homodimeric protein consisting of 70 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human MIP-1 alpha chain was expressed in E. coli.
Description: Recombinant MIP-1 alpha is a disulfide-linked homodimeric protein consisting of 70 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human MIP-1 alpha chain was expressed in E. coli.
Description: Recombinant MIP-1 beta is a disulfide-linked homodimeric protein consisting of 70 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human MIP-1 beta chain was expressed in E. coli.
Description: Recombinant MIP-1 beta is a disulfide-linked homodimeric protein consisting of 70 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human MIP-1 beta chain was expressed in E. coli.
Macrophage Inflammatory Protein 1 Alpha (MIP1a) Antibody
Description: Macrophage Inflammatory Protein-1 alpha His Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7820 Dalton with an amino-terminal hexahistidine tag. ;The MIP-1a-His is purified by proprietary chromatographic techniques.
Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Description: MIP-3 Alpha Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 8 kDa. ;The MIP-3a is purified by proprietary chromatographic techniques.
Recombinant Macrophage Inflammatory Protein Related Protein 1 (MRP1)
The same pattern of activation was observed in the level of protein in the cortex and thalamus, where the strongest activation was observed one day after CCI; However, we did not observe changes in the thalamus CCL3. CCR1 and CCR5 analysis showed upregulation of mRNA expression of both receptors in all tested brain structures, especially in the early phases of post injury (24 hours, 4 days and 7 days).
