Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation.
Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation.
Chronic inflammation of the adipose tissue contributes to obesity induced insulin resistance. Unfortunately, the potential molecular mechanisms of systemic inflammation and obesity-related metabolic disorders remain complicated. Here, we report that is not active rhomboid-like protein 2 (iRhom2) is increased in obese mice with adipose inflammation.Mice with the elimination of background iRhom2 in C57BL / 6J mice without this gene deletion (control), and mice deficient in only iRhom2 on myeloid cells were fed standard chow diet (SCD) or a high-fat diet (HFD; 60% fat calories).
Then the adipose tissue or bone marrow cells were isolated for more detection.After 16 weeks on a high fat diet (HFD), obesity, chronic inflammation in adipose tissue and insulin resistance were markedly reduced in iRhom2 KO (iRhom2 KO) mice, whereas these parameters exaggerated -besarkan in iRhom2 overactive mice. Adverse effects of iRhom2 in adipose inflammation and related pathologies are determined in db / db mice. We further show that, in response to an HFD mice, iRhom2 knockouts and mice with deletion only in myeloid cells showed less severe inflammation of the adipose tissue and insulin resistance compared to the control group.
By contrast, transplantation of bone marrow cells from normal mice KO mice iRhom2 severe systemic release of inflammatory and metabolic dysfunction after HFD ingestion.We iRhom2 identified as a key regulator that promotes obesity-related metabolic disorders. IRhom2 loss of macrophages in adipose tissue indirectly restrain inflammation and insulin resistance by blocking crosslinks between macrophages and adipocytes. Therefore, iRhom2 may be a therapeutic target for obesity-induced metabolic dysfunction.
Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation.
Changes in macrophage inflammatory protein-1 (MIP-1) expression family members caused by traumatic brain injury in rats.
A deep knowledge of the profound immunological response caused by traumatic brain injury (TBI) raises the possibility of new therapeutic interventions. No studies have highlighted the important role of C-C ligand motif in the development of nerve inflammation after brain injury;
However, the participation of family members macrophage inflammatory protein-1 (MIP-1) in this phenomenon is still undefined. Therefore, the aim of our study was to evaluate changes in inflammatory protein-1 macrophages (MIP-1) family (CCL3, CCl4, and CCL9) and their receptors (CCR1 and CCR5) in a mouse model of TBI (induced by controlled impact cortical (CCI)). We also investigated the patterns of activation of immunological cells (such as neutrophils, microglia and astroglia), which on the one hand express CCR1 / CCR5, and on the other hand may be a source of chemokines tested in brain injury.
We investigated the changes in mRNA (RT-qPCR) and / or protein (ELISA and Western blot) expression in brain structures (cortex, hippocampus, thalamus, and striatum) at points different time (24 hours, 4 days, 7 days, 2 weeks, and / or 5 weeks) after trauma. Studies of time-course revealed upregulation of mRNA expression of all members of MIP-1 family (CCL3, CCl4, and CCL9) in all tested brain structures, especially in the early stages after injury.
Macrophage Inflammatory Protein 1 Gamma (MIP1g) Antibody
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP1g in the samples is then determined by comparing the OD of the samples to the standard curve.
Rat MIP1g(Macrophage Inflammatory Protein 1 Gamma) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP1g in the samples is then determined by comparing the OD of the samples to the standard curve.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Macrophage Inflammatory Protein 1 gamma, Murine (MIP-1g) (APC)
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MIP1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MIP1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MIP1g in the samples is then determined by comparing the OD of the samples to the standard curve.
Mouse MIP1g(Macrophage Inflammatory Protein 1 Gamma) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MIP1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MIP1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MIP1g in the samples is then determined by comparing the OD of the samples to the standard curve.
Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Macrophage Inflammatory Protein 1 Gamma-CCL9 ； MIP-1γ-
The same pattern of activation was observed in the level of protein in the cortex and thalamus, where the strongest activation was observed one day after CCI; However, we did not observe changes in the thalamus CCL3. CCR1 and CCR5 analysis showed upregulation of mRNA expression of both receptors in all tested brain structures, especially in the early phases of post injury (24 hours, 4 days and 7 days).
