Suppressors of Cytokine Signaling (SOCS)1 and SOCS3 Proteins Are Mediators of Interleukin-10 Modulation of Inflammatory Responses Induced by Chlamydia muridarum and Its Major Outer Membrane Protein (MOMP) in Mouse J774 Macrophages
Suppressors of Cytokine Signaling (SOCS)1 and SOCS3 Proteins Are Mediators of Interleukin-10 Modulation of Inflammatory Responses Induced by Chlamydia muridarum and Its Major Outer Membrane Protein (MOMP) in Mouse J774 Macrophages
The immunopathology of chlamydial disease exacerbated by the broad spectrum of inflammatory mediators, which we reported inhibited by IL-10 in macrophages. However, chlamydial protein that induces part inflammatory mediators and the mechanisms that IL-10 inhibits them is unknown. We hypothesize that the primary Chlamydia outer membrane protein (MOMP) mediate the pathogenesis of the disease, as well as the suppressor of cytokine signaling (SOCS) 1 and SOCS3 protein is the mediator of the inhibition of IL-10 action. Our hypothesis was tested by exposing mice to a stimulant J774 macrophages chlamydia (Chlamydia muridarum life and MOMP) with and without IL-10. MOMP significantly due to several inflammatory mediators (IL-6, IL-12p40, CCL5, CXCL10), which are dose-dependent inhibited by IL-10.
stimulants due to mRNA transcripts chlamydial gene and protein expression of SOCS1 and SOCS3, with more SOCS3 expression. Specifically, IL-10 reciprocally regulated their expression by reducing SOCS1 and SOCS3 increase. specific barriers MAPK pathways revealed that p38, JNK, and MEK1 / 2, which is required to drive the inflammatory mediators and SOCS1 and SOCS3. stimulant chlamydia trigger M1 pro-inflammatory phenotype evident by nos2 improved (M1 marker) expression, which is affected by IL-10 towards a more M2 anti-inflammatory phenotype with increased expression of mrc1 and arg1 (M2 marker) and reduced the ratio of SOCS1 / SOCS3.
Neutralization of endogenous produced IL-10 plus secretion of inflammatory mediators, reduce the expression of SOCS3, and slanted chlamydia M1 to M2 phenotype. Inhibition of the proteasome degradation increased TNF but decreased IL-10, CCL5 and CXCL10 secretion by pressing SOCS1 and SOCS3 expression and dysregulating STAT1 and STAT3 transcription factor them. Our data indicate that SOCS1 and SOCS3 is a regulator of IL-10 inhibitory action and underlined the SOCS protein as a therapeutic target for IL-10 control inflammation for Chlamydia and other bacterial inflammatory disease.
Suppressors of Cytokine Signaling (SOCS)1 and SOCS3 Proteins Are Mediators of Interleukin-10 Modulation of Inflammatory Responses Induced by Chlamydia muridarum and Its Major Outer Membrane Protein (MOMP) in Mouse J774 Macrophages
Age-dependent changes in the immunoreactivity of macrophage inflammatory protein-3α and CCR6 receptor in the hippocampus gerbils
Neuroinflammation is the main characteristic of the aging brain. During normal aging, macrophage inflammatory protein-3α (MIP-3α) and the receptor C-C chemokine receptor type 6 (CCR6) serves an important role in neuroinflammatory processes in the brain. The purpose of this study was to investigate the age-dependent changes in immunoreactivity of MIP-3α and CCR6 in gerbils in the postnatal hippocampus (PM) 1, 6, 12 and 24 through immunohistochemistry.
In the group of PM 1, both MIP-3α and CCR6 immunoreactivity was observed mainly in pyramidale strata in the right hippocampus and in the granule cell layer of the dentate gyrus. In PM 6 and PM 12 group, MIP-3α in pyramidale strata and layers of granule cells was decreased compared with PM1, and CCR6 immunoreactivity in both layers were sketchy. In the group of PM 24, the expression of MIP-3α in pyramidale strata and granule cell layer is higher than in the group of PM 1 and CCR6 immunoreactivity in both layers was increased compared with the PM 12 group;
Macrophage Inflammatory Protein 3 Alpha (MIP3a) Antibody
Description: Recombinant Human Active Macrophage Inflammatory Protein 3 Alpha (MIP3a) expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells
Active Macrophage Inflammatory Protein 3 Alpha (MIP3a)
Description: Recombinant MIP-1 alpha is a disulfide-linked homodimeric protein consisting of 70 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human MIP-1 alpha chain was expressed in E. coli.
Description: Recombinant MIP-1 alpha is a disulfide-linked homodimeric protein consisting of 70 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human MIP-1 alpha chain was expressed in E. coli.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Bovine Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Canine Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Canine Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Canine Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Canine Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma or other biological fluids.
Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma or other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Macrophage Inflammatory Protein 3 Alpha (MIP3a) Monoclonal Antibody (Human)
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma and other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma and other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma and other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a)CLIA Kit
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma and other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) CLIA Kit
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a)Serum, plasma and other biological fluids
Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma and other biological fluids.
Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma and other biological fluids.
Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma and other biological fluids.
Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma and other biological fluids.
Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.
Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Bovine Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
However, it decreased compared to the PM group 1. In conclusion, MIP-3α and CCR6 altered immunoreactivity in the hippocampus during the normal aging process. Results of this study suggest that age-dependent changes of MIP-3α and CCR6 may be associated with age-related neuroinflammation in the hippocampus.
